• Western blot analysis of various lysates using USP14 Rabbit polyclonal antibody (STJ119067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 2min.

Anti-USP14 antibody (400-500) (STJ119067)

SKU:
STJ119067

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP14 (400-500) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP14
Gene ID: 9097
Uniprot ID: UBP14_HUMAN
Immunogen Region: 400-500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human USP14 (NP_005142.1).
Immunogen Sequence: KYEPFSFADDIGSNNCGYYD LQAVLTHQGRSSSSGHYVSW VKRKQDEWIKFDDDKVSIVT PEDILRLSGGGDWHIAYVLL YGPRRVEIMEEESEQ
Function Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 14
Deubiquitinating Enzyme 14
Ubiquitin Thioesterase 14
Ubiquitin-Specific-Processing Protease 14
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-9758274
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 14 antibody
Anti-Deubiquitinating Enzyme 14 antibody
Anti-Ubiquitin Thioesterase 14 antibody
Anti-Ubiquitin-Specific-Processing Protease 14 antibody
Anti-USP14 antibody
Anti-TGT antibody

Information sourced from Uniprot.org

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