| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-USP13 (300-350 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). Use at a dilution of 1:200 to 1:2000. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
USP13 |
| Gene ID: |
8975 |
| Uniprot ID: |
UBP13_HUMAN |
| Immunogen Region: |
300-350 aa |
| Specificity: |
Specific for USP13. |
| Immunogen: |
A synthetic peptide from aa region 300-350 of human USP13 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen shares 93% identity with mouse and rat sequences. |
| Post Translational Modifications | Phosphorylated by AURKB at Ser-114.leading to stabilization of cell cycle proteins such as SKP2 and AURKB, but not MCL1. |
| Function | Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy, endoplasmic reticulum-associated degradation (ERAD), cell cycle progression or DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Alternatively, forms with NEDD4 a deubiquitination complex, which subsequently stabilizes VPS34 to promote autophagy. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Regulates the cell cycle progression by stabilizing cell cycle proteins such as SKP2 and AURKB. In addition, plays an important role in maintaining genomic stability and in DNA replication checkpoint activation via regulation of RAP80 and TOPBP1. Deubiquitinates the multifunctional protein HMGB1 and subsequently drives its nucleocytoplasmic localization and its secretion. Positively regulates type I and type II interferon signalings by deubiquitinating STAT1 but negatively regulates antiviral response by deubiquitinating STING1. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 13Deubiquitinating Enzyme 13Isopeptidase T-3Isot-3Ubiquitin Thioesterase 13Ubiquitin-Specific-Processing Protease 13 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-8948751 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 13 antibodyAnti-Deubiquitinating Enzyme 13 antibodyAnti-Isopeptidase T-3 antibodyAnti-Isot-3 antibodyAnti-Ubiquitin Thioesterase 13 antibodyAnti-Ubiquitin-Specific-Processing Protease 13 antibodyAnti-USP13 antibodyAnti-ISOT3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
