Anti-USH1C/Harmonin antibody (N-Term) (STJ70512)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-USH1C/Harmonin (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 75kDa band observed in lysates of cell line HEK293 (calculated MW of 62.2kDa according to NP_005700.2). Recommended concentration: 0.1-0.3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | USH1C |
| Gene ID | 10083 |
| Uniprot ID | USH1C_HUMAN |
| Accession Number | NP_005700.2; NP_710142.1 |
| Immunogen Region | N-Term |
| Immunogen Sequence | DRKVAREFRHKVD |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_005700.2 and NP_710142.1). |
Additional Info
| Function | Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal development and maintenance of cochlear hair cell bundles. As part of the intermicrovillar adhesion complex/IMAC plays a role in brush border differentiation, controlling microvilli organization and length. Probably plays a central regulatory role in the assembly of the complex, recruiting CDHR2, CDHR5 and MYO7B to the microvilli tips. |
| Protein Name | HarmoninAntigen Ny-Co-38/Ny-Co-37Autoimmune Enteropathy-Related Antigen Aie-75Protein Pdz-73Renal Carcinoma Antigen Ny-Ren-3Usher Syndrome Type-1c Protein |
| Database Links | Reactome: R-HSA-9662360Reactome: R-HSA-9662361 |
| Cellular Localisation | CytoplasmCytosolCytoskeletonCell ProjectionMicrovillusColocalizes With F-ActinDetected At The Tip Of Cochlear Hair Cell StereociliaEnriched In Microvilli Of The Intestinal Brush Border |
| Alternative Antibody Names | Anti-Harmonin antibodyAnti-Antigen Ny-Co-38/Ny-Co-37 antibodyAnti-Autoimmune Enteropathy-Related Antigen Aie-75 antibodyAnti-Protein Pdz-73 antibodyAnti-Renal Carcinoma Antigen Ny-Ren-3 antibodyAnti-Usher Syndrome Type-1c Protein antibodyAnti-USH1C antibodyAnti-AIE75 antibody |
Information sourced from Uniprot.org