• Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells using 1 Mu g UQCRC2 antibody (STJ26050). Western blot was performed from the immunoprecipitate using UQCRC2 antibody (STJ26050) at a dilution of 1:1000.
  • Immunohistochemistry analysis of UQCRC2 in paraffin-embedded human colon using UQCRC2 Rabbit polyclonal antibody (STJ26050) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of UQCRC2 in paraffin-embedded rat kidney using UQCRC2 Rabbit polyclonal antibody (STJ26050) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using UQCRC2 Rabbit polyclonal antibody (STJ26050) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-UQCRC2 antibody (224-453) (STJ26050)

SKU:
STJ26050

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UQCRC2 (224-453) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UQCRC2
Gene ID: 7385
Uniprot ID: QCR2_HUMAN
Immunogen Region: 224-453
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 224-453 of human UQCRC2 (NP_003357.2).
Immunogen Sequence: GVSHPVLKQVAEQFLNMRGG LGLSGAKANYRGGEIREQNG DSLVHAAFVAESAVAGSAEA NAFSVLQHVLGAGPHVKRGS NTTSHLHQAVAKATQQPFDV SAFNASYSDSGLFGIYTISQ ATAAGDVIKAAYNQVKTIAQ GNLSNTDVQAAKNKLKAGYL MSVESSECFLEEVGSQALVA GSYMPPSTVLQQIDSVANAD IINAAKKFVSGQKSMAASGN LGHTPFVDEL
Function Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties. May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable).
Protein Name Cytochrome B-C1 Complex Subunit 2 - Mitochondrial
Complex Iii Subunit 2
Core Protein Ii
Ubiquinol-Cytochrome-C Reductase Complex Core Protein 2
Database Links Reactome: R-HSA-611105
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Cytochrome B-C1 Complex Subunit 2 - Mitochondrial antibody
Anti-Complex Iii Subunit 2 antibody
Anti-Core Protein Ii antibody
Anti-Ubiquinol-Cytochrome-C Reductase Complex Core Protein 2 antibody
Anti-UQCRC2 antibody

Information sourced from Uniprot.org

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