Anti-UQCRC2 antibody (224-453) (STJ26050)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal UQCRC2 (224-453) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC: 1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your speci |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UQCRC2 |
| Gene ID | 7385 |
| Uniprot ID | QCR2_HUMAN |
| Immunogen Region | 224-453 |
| Immunogen Sequence | GVSHPVLKQVAEQFLNMRGG LGLSGAKANYRGGEIREQNG DSLVHAAFVAESAVAGSAEA NAFSVLQHVLGAGPHVKRGS NTTSHLHQAVAKATQQPFDV SAFNASYSDSGLFGIYTISQ ATAAGDVIKAAYNQVKTIAQ GNLSNTDVQAAKNKLKAGYL MSVESSECFLEEVGSQALVA GSYMPPSTVLQQIDSVANAD IINAAKKFVSGQKSMAASGN LGHTPFVDEL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 224-453 of human UQCRC2 (NP_003357.2). |
Additional Info
| Function | Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties. May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable). |
| Protein Name | Cytochrome B-C1 Complex Subunit 2 - MitochondrialComplex Iii Subunit 2Core Protein IiUbiquinol-Cytochrome-C Reductase Complex Core Protein 2 |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-9837999Reactome: R-HSA-9865881 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Cytochrome B-C1 Complex Subunit 2 - Mitochondrial antibodyAnti-Complex Iii Subunit 2 antibodyAnti-Core Protein Ii antibodyAnti-Ubiquinol-Cytochrome-C Reductase Complex Core Protein 2 antibodyAnti-UQCRC2 antibody |
Information sourced from Uniprot.org