Anti-UPF3A/RENT3A antibody (Internal) (STJ72041)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-UPF3A/RENT3A (Internal) is suitable for use in ELISA research applications. |
| Applications | Pep-ELISA |
| Host | Goat |
| Reactivity | Human/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 48kDa band in lysates of cell lines HeLa and Kelly after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in t |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | UPF3A |
| Gene ID | 65110 |
| Uniprot ID | REN3A_HUMAN |
| Accession Number | NP_075387.1; NP_542418.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | KETDKQKKIAEKE |
| Specificity | This antibody is expected to recognize both isoforms (NP_075387.1; NP_542418.1). |
Additional Info
| Function | Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation. |
| Protein Name | Regulator Of Nonsense Transcripts 3aNonsense Mrna Reducing Factor 3aUp-Frameshift Suppressor 3 Homolog AHupf3 |
| Database Links | Reactome: R-HSA-9010553Reactome: R-HSA-975957 Q9H1J1-2 |
| Cellular Localisation | NucleusCytoplasmShuttling Between The Nucleus And The Cytoplasm |
| Alternative Antibody Names | Anti-Regulator Of Nonsense Transcripts 3a antibodyAnti-Nonsense Mrna Reducing Factor 3a antibodyAnti-Up-Frameshift Suppressor 3 Homolog A antibodyAnti-Hupf3 antibodyAnti-UPF3A antibodyAnti-RENT3A antibodyAnti-UPF3 antibody |
Information sourced from Uniprot.org