Anti-UPF3A antibody (327-476) (STJ118338)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal UPF3A (327-476) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UPF3A |
| Gene ID | 65110 |
| Uniprot ID | REN3A_HUMAN |
| Immunogen Region | 327-476 |
| Immunogen Sequence | EGSLEEPQETSHSGSDKEHR DVERSQEQESEAQRYHVDDG RRHRAHHEPERLSRRSEDEQ RWGKGPGQDRGKKGSQDSGA PGEAMERLGRAQRCDDSPAP RKERLANKDRPALQLYDPGA RFRARECGGNRRICKAEGSG TGPEKREEAE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 327-476 of human UPF3A (NP_075387.1). |
Additional Info
| Tissue Specificity | Isoform 1 is strongly expressed in testis, uterus, muscle, fetal brain and spinal cord. Isoform 2 is strongly expressed in fetal brain and spinal cord. |
| Function | Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation. |
| Protein Name | Regulator Of Nonsense Transcripts 3aNonsense Mrna Reducing Factor 3aUp-Frameshift Suppressor 3 Homolog AHupf3 |
| Database Links | Reactome: R-HSA-9010553Reactome: R-HSA-975957 Q9H1J1-2 |
| Cellular Localisation | NucleusCytoplasmShuttling Between The Nucleus And The Cytoplasm |
| Alternative Antibody Names | Anti-Regulator Of Nonsense Transcripts 3a antibodyAnti-Nonsense Mrna Reducing Factor 3a antibodyAnti-Up-Frameshift Suppressor 3 Homolog A antibodyAnti-Hupf3 antibodyAnti-UPF3A antibodyAnti-RENT3A antibodyAnti-UPF3 antibody |
Information sourced from Uniprot.org