Anti-UNC5B antibody (629-643) (STJ72651)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-UNC5B (629-643) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 90kDa band observed in Human Bone Marrow lysates (calculated MW of 104kDa according to NP_734465.2). Some minor background is detected and is blocked by the immunizing peptide. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | UNC5B |
| Gene ID | 219699 |
| Uniprot ID | UNC5B_HUMAN |
| Accession Number | NP_734465.2 |
| Immunogen Region | 629-643 |
| Immunogen Sequence | SARDWIFQLKTQAHQ |
Additional Info
| Post Translational Modifications | Phosphorylated on cytoplasmic tyrosine residues. Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis. Palmitoylation is required for pro-apoptotic activity, but not for location at lipid rafts. |
| Function | Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Functions as a netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity. |
| Protein Name | Netrin Receptor Unc5bProtein Unc-5 Homolog 2Protein Unc-5 Homolog BP53-Regulated Receptor For Death And Life Protein 1P53rdl1 |
| Database Links | Reactome: R-HSA-373752Reactome: R-HSA-418886Reactome: R-HSA-418889 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftAssociated With Lipid Rafts |
| Alternative Antibody Names | Anti-Netrin Receptor Unc5b antibodyAnti-Protein Unc-5 Homolog 2 antibodyAnti-Protein Unc-5 Homolog B antibodyAnti-P53-Regulated Receptor For Death And Life Protein 1 antibodyAnti-P53rdl1 antibodyAnti-UNC5B antibodyAnti-P53RDL1 antibodyAnti-UNC5H2 antibodyAnti-UNQ1883 antibodyAnti-PRO4326 antibody |
Information sourced from Uniprot.org