Anti-UNC5B antibody (290-377) (STJ117177)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal UNC5B (290-377) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UNC5B |
| Gene ID | 219699 |
| Uniprot ID | UNC5B_HUMAN |
| Immunogen Region | 290-377 |
| Immunogen Sequence | FQKTACTTICPVDGAWTEWS KWSACSTECAHWRSRECMAP PPQNGGRDCSGTLLDSKNCT DGLCMQNKKTLSDPNSHLLE ASGDAALY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 290-377 of human UNC5B (NP_734465.2). |
Additional Info
| Tissue Specificity | Highly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues. |
| Post Translational Modifications | Phosphorylated on cytoplasmic tyrosine residues. Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis. Palmitoylation is required for pro-apoptotic activity, but not for location at lipid rafts. |
| Function | Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Functions as a netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity. |
| Protein Name | Netrin Receptor Unc5bProtein Unc-5 Homolog 2Protein Unc-5 Homolog BP53-Regulated Receptor For Death And Life Protein 1P53rdl1 |
| Database Links | Reactome: R-HSA-373752Reactome: R-HSA-418886Reactome: R-HSA-418889 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftAssociated With Lipid Rafts |
| Alternative Antibody Names | Anti-Netrin Receptor Unc5b antibodyAnti-Protein Unc-5 Homolog 2 antibodyAnti-Protein Unc-5 Homolog B antibodyAnti-P53-Regulated Receptor For Death And Life Protein 1 antibodyAnti-P53rdl1 antibodyAnti-UNC5B antibodyAnti-P53RDL1 antibodyAnti-UNC5H2 antibodyAnti-UNQ1883 antibodyAnti-PRO4326 antibody |
Information sourced from Uniprot.org