• Immunofluorescence analysis of NIH-3T3 cells using UMPS Rabbit polyclonal antibody (STJ115216) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using UMPS Rabbit polyclonal antibody (STJ115216) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-UMPS antibody (100-260) (STJ115216)

SKU:
STJ115216

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UMPS (100-260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UMPS
Gene ID: 7372
Uniprot ID: UMPS_HUMAN
Immunogen Region: 100-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-260 of human UMPS (NP_000364.1).
Immunogen Sequence: TKDYGTKRLVEGTINPGETC LIIEDVVTSGSSVLETVEVL QKEGLKVTDAIVLLDREQGG KDKLQAHGIRLHSVCTLSKM LEILEQQKKVDAETVGRVKR FIQENVFVAANHNGSPLSIK EAPKELSFGARAELPRIHPV ASKLLRLMQKKETNLCLSAD V
Function Bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis, orotate phosphoribosyltransferase (OPRT), which converts orotate to orotidine-5'-monophosphate (OMP), and orotidine-5'-monophosphate decarboxylase (ODC), the terminal enzymatic reaction that decarboxylates OMP to uridine monophosphate (UMP).
Protein Name Uridine 5'-Monophosphate Synthase
Ump Synthase Includes - Orotate Phosphoribosyltransferase
Oprt
Oprtase - Orotidine 5'-Phosphate Decarboxylase
Odc
Ompd
Ompdecase
Database Links Reactome: R-HSA-500753
Alternative Antibody Names Anti-Uridine 5'-Monophosphate Synthase antibody
Anti-Ump Synthase Includes - Orotate Phosphoribosyltransferase antibody
Anti-Oprt antibody
Anti-Oprtase - Orotidine 5'-Phosphate Decarboxylase antibody
Anti-Odc antibody
Anti-Ompd antibody
Anti-Ompdecase antibody
Anti-UMPS antibody
Anti-OK antibody
Anti-SW-cl.21 antibody

Information sourced from Uniprot.org

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