Anti-ULK3 antibody (445-458) (STJ72686)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ULK3 (445-458) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Pig/Dog |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50kDa band observed in Human Brain (Cerebellum, Cerebral Cortex, Hippocampus) lysates (calculated MW of 53.4kDa according to NP_001092906.1). Recommended concentration: 1-3µg/ml |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ULK3 |
| Gene ID | 25989 |
| Uniprot ID | ULK3_HUMAN |
| Accession Number | NP_001092906.1 |
| Immunogen Region | 445-458 |
| Immunogen Sequence | KMRESRWEADTLDK |
Additional Info
| Post Translational Modifications | Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU. |
| Function | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. |
| Protein Name | Serine/Threonine-Protein Kinase Ulk3Unc-51-Like Kinase 3 |
| Database Links | Reactome: R-HSA-5632684 |
| Cellular Localisation | CytoplasmLocalizes To Pre-Autophagosomal Structure During Cellular Senescence |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Ulk3 antibodyAnti-Unc-51-Like Kinase 3 antibodyAnti-ULK3 antibody |
Information sourced from Uniprot.org