• Western blot analysis of various lysates using UIMC1 Rabbit polyclonal antibody (STJ116723) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UIMC1 antibody (449-719) (STJ116723)

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STJ116723

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UIMC1 (449-719) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UIMC1
Gene ID: 51720
Uniprot ID: UIMC1_HUMAN
Immunogen Region: 449-719
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 449-719 of human UIMC1 (NP_057374.3).
Immunogen Sequence: QLSSSETFDLEREVSPGSRD ILDGVRIIMADKEVGNKEDA EKEVAISTFSSSNQVSCPLC DQCFPPTKIERHAMYCNGLM EEDTVLTRRQKEAKTKSDSG TAAQTSLDIDKNEKCYLCKS LVPFREYQCHVDSCLQLAKA DQGDGPEGSGRACSTVEGKW QQRLKNPKEKGHSEGRLLSF LEQSEHKTSDADIKSSETGA FRVPSPGMEEAGCSREMQSS FTRRDLNESPVKSFVSISE
Tissue Specificity Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
Post Translational Modifications Sumoylated. Phosphorylated upon DNA damage by ATM or ATR.
Function Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B.the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
Protein Name Brca1-A Complex Subunit Rap80
Receptor-Associated Protein 80
Retinoid X Receptor-Interacting Protein 110
Ubiquitin Interaction Motif-Containing Protein 1
Database Links Reactome: R-HSA-5689901
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-69473
Cellular Localisation Nucleus
Localizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs)
Alternative Antibody Names Anti-Brca1-A Complex Subunit Rap80 antibody
Anti-Receptor-Associated Protein 80 antibody
Anti-Retinoid X Receptor-Interacting Protein 110 antibody
Anti-Ubiquitin Interaction Motif-Containing Protein 1 antibody
Anti-UIMC1 antibody
Anti-RAP80 antibody
Anti-RXRIP110 antibody

Information sourced from Uniprot.org

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