Anti-NASP antibody (180-449) (STJ29018)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NASP (180-449) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NASP |
| Gene ID | 4678 |
| Uniprot ID | NASP_HUMAN |
| Immunogen Region | 180-449 |
| Immunogen Sequence | WDMLDLAKIIFKRQETKEAQ LYAAQAHLKLGEVSVESENY VQAVEEFQSCLNLQEQYLEA HDRLLAETHYQLGLAYGYNS QYDEAVAQFSKSIEVIENRM AVLNEQVKEAEGSSAEYKKE IEELKELLPEIREKIEDAKE SQRSGNVAELALKATLVESS TSGFTPGGGGSSVSMIASRK PTDGASSSNCVTDISHLVRK KRKPEEESPRKDDAKKAKQE PEVNGGSGDAVPSGNEVSE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2). |
Additional Info
| Tissue Specificity | Isoform 1 is testis- and sperm-specific. |
| Function | Component of the histone chaperone network. Binds and stabilizes histone H3-H4 not bound to chromatin to maintain a soluble reservoir and modulate degradation by chaperone-mediated autophagy. Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. Isoform 1: Stabilizes soluble histone H3-H4. Isoform 2: Stabilizes soluble histone H3-H4. |
| Protein Name | Nuclear Autoantigenic Sperm ProteinNasp |
| Database Links | |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Nuclear Autoantigenic Sperm Protein antibodyAnti-Nasp antibodyAnti-NASP antibody |
Information sourced from Uniprot.org