• Western blot analysis of extracts of various cell lines, using Uhrf2 antibody (STJ115606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-UHRF2 antibody (76-359) (STJ115606)

SKU:
STJ115606

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Uhrf2 (76-359) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Uhrf2
Gene ID: 109113
Uniprot ID: UHRF2_MOUSE
Immunogen Region: 76-359
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 76-359 of mouse Uhrf2 (NP_659122.2).
Immunogen Sequence: DSSLPSTSKQNDAQVKPSSH NPPKVKKTARGGSSSQPSTS ARTCLIDPGFGLYKVNELVD ARDVGLGAWFEAHIHSVTRA SDGHSRGKTPLKNGSSYKRT NGNVNHNSKENTNKLDNVPS TSNSDSVAADEDVIYHIEYD EYPESGILEMNVKDLRPRAR TILKWNELNVGDVVMVNYNV ENPGKRGFWYDAEITTLKTI SRTKKEVRVKVFLGGSEGTL NDCRVMSVDEIFKIEKPGA
Tissue Specificity Mostly detected in several tissues, including the thymus, spleen, lung, adrenal gland, and ovary. In addition, found in several tissues in the brain (cerebellum, hippocampus, and cerebral cortex).
Post Translational Modifications May be autoubiquitinated.which may lead to proteasomal degradation. Phosphorylated. Phosphorylation may be mediated by CDK2. Autosumoylated.
Function E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair. Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them. This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression. Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thus inducing G1 arrest. Ubiquitinates also PCNP leading to its degradation by the proteasome. Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteasomal degradation. Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation. Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation.
Protein Name E3 Ubiquitin-Protein Ligase Uhrf2
Nirf
Np95-Like Ring Finger Protein
Nuclear Protein 97
Nuclear Zinc Finger Protein Np97
Ring-Type E3 Ubiquitin Transferase Uhrf2
Ubiquitin-Like Phd And Ring Finger Domain-Containing Protein 2
Ubiquitin-Like-Containing Phd And Ring Finger Domains Protein 2
Database Links Reactome: R-MMU-3899300
Cellular Localisation Nucleus
Chromosome
Enriched At Genomic Loci That Are Enriched For 5-Hydroxy-Methylcytosine (5hmc)
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Uhrf2 antibody
Anti-Nirf antibody
Anti-Np95-Like Ring Finger Protein antibody
Anti-Nuclear Protein 97 antibody
Anti-Nuclear Zinc Finger Protein Np97 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Uhrf2 antibody
Anti-Ubiquitin-Like Phd And Ring Finger Domain-Containing Protein 2 antibody
Anti-Ubiquitin-Like-Containing Phd And Ring Finger Domains Protein 2 antibody
Anti-Uhrf2 antibody
Anti-Nirf antibody

Information sourced from Uniprot.org

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