| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-E3 ubiquitin-protein ligase UHRF1 is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
1H9-D3-E11 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2b |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UHRF1 |
| Gene ID: |
29128 |
| Uniprot ID: |
UHRF1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of UHRF1 and does not cross-react with related proteins |
| Immunogen: |
Purified recombinant human UHRF1 protein fragments expressed in E.coil. |
| Post Translational Modifications | Phosphorylation at Ser-298 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome. Ubiquitinated.which leads to proteasomal degradation. Autoubiquitinated.interaction with USP7 leads to deubiquitination and prevents degradation. Ubiquitination and degradation takes place during M phase, when phosphorylation at Ser-639 prevents interaction with USP7 and subsequent deubiquitination. Polyubiquitination may be stimulated by DNA damage. |
| Function | Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF1 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Uhrf1Inverted Ccaat Box-Binding Protein Of 90 KdaNuclear Protein 95Nuclear Zinc Finger Protein Np95Hunp95Hnp95Ring Finger Protein 106Ring-Type E3 Ubiquitin Transferase Uhrf1Transcription Factor Icbp90Ubiquitin-Like Phd And Ring Finger Domain-Containing Protein 1Huhrf1Ubiquitin-Like-Containing Phd And Ring Finger Domains Protein 1 |
| Database Links | Reactome: R-HSA-5334118 |
| Cellular Localisation | NucleusAssociatedThrough The Ydg Domain (Also Called Sra Domain)With Replicating Dna From Early To Late S PhaseIncluding At Replicating Pericentric HeterochromatinAlso Localizes To Euchromatic RegionsIn Non-S-Phase CellsHomogenously Distributed Through The Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Uhrf1 antibodyAnti-Inverted Ccaat Box-Binding Protein Of 90 Kda antibodyAnti-Nuclear Protein 95 antibodyAnti-Nuclear Zinc Finger Protein Np95 antibodyAnti-Hunp95 antibodyAnti-Hnp95 antibodyAnti-Ring Finger Protein 106 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Uhrf1 antibodyAnti-Transcription Factor Icbp90 antibodyAnti-Ubiquitin-Like Phd And Ring Finger Domain-Containing Protein 1 antibodyAnti-Huhrf1 antibodyAnti-Ubiquitin-Like-Containing Phd And Ring Finger Domains Protein 1 antibodyAnti-UHRF1 antibodyAnti-ICBP90 antibodyAnti-NP95 antibodyAnti-RNF106 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
