• Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.
  • Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 (STJA0003820, 1:500, red) and anti-Neurofilament H ( cat. STJA0003698, 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.
  • Immunofluorescence of a section of rat hippocampus showing specific labeling of UCHL1 (STJA0003820, 1:5000, green) in cell bodies and dendrites of neurons, and specific labeling of FOX3 (red). The blue is DAPI staining of nuclear DNA.
  • Immunolabeling of HEK 293 cells labeled with anti-UCHL1 antibody (STJA0003820, green, 1:500) and anti-neuron specific enolase antibody (1520-NSE, red, 1:500). The blue is staining nuclear DNA.

Anti-UCHL1 antibody (Full Length) [BH7] (STJA0003820)

SKU:
STJA0003820-100

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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: Bovine/Human/Mouse/Pig/Rat/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-UCHL1 (Full Length) is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: BH7
Conjugation: Unconjugated
Isotype: IgG1
Formulation: 100 ul in PBS + 50% Glycerol and 5 mm Sodium Azide
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:5000
IHC 1:500-1:5000
ICC 1:500-1:2000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UCHL1
Gene ID: 7345
Uniprot ID: UCHL1_HUMAN
Immunogen Region: Full Length
Immunogen: Recombinant full length human UCHL1 purification from E. coli.
Tissue Specificity Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
Post Translational Modifications O-glycosylated.
Function Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways. Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production. Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes. Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis. Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators. Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins. Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1
Uch-L1
Neuron Cytoplasmic Protein 9.5
Pgp 9.5
Pgp9.5
Ubiquitin Thioesterase L1
Database Links Reactome: R-HSA-5689603
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Lipid-Anchor
About 30% Of Total Uchl1 Is Associated With Membranes In Brain
Localizes Near And/Or Within Mitochondria To Potentially Interact With Mitochondrial Proteins
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 antibody
Anti-Uch-L1 antibody
Anti-Neuron Cytoplasmic Protein 9.5 antibody
Anti-Pgp 9.5 antibody
Anti-Pgp9.5 antibody
Anti-Ubiquitin Thioesterase L1 antibody
Anti-UCHL1 antibody

Information sourced from Uniprot.org

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