Anti-Ubiquitin Conjugating Enzyme E2 A/B antibody [R87-4N-5] (STJA0035504)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from UBE2B
General Information
| Short Description | Rabbit monoclonal anti-Ubiquitin Conjugating Enzyme E2 A/B for use in WB, IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R87-4N-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:100-1:200IF 1:50-1:200 |
| Formulation | Liquid in PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | UBE2B |
| Gene ID | 7320 |
| Uniprot ID | UBE2B_HUMAN |
| Immunogen | A synthesized peptide derived from UBE2B |
Additional Info
| Function | E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. May play a role in DNA repair. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. In association with the E3 enzyme UBR4, is involved in N-end rule-dependent protein degradation. May be involved in neurite outgrowth. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 BE2 Ubiquitin-Conjugating Enzyme BRad6 Homolog BHr6bHhr6bUbiquitin Carrier Protein BUbiquitin-Conjugating Enzyme E2-17 KdaUbiquitin-Protein Ligase B |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-983168 |
| Cellular Localisation | Cell MembraneNucleusIn Peripheral NeuronsExpressed Both At The Plasma Membrane And In Nuclei |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 B antibodyAnti-E2 Ubiquitin-Conjugating Enzyme B antibodyAnti-Rad6 Homolog B antibodyAnti-Hr6b antibodyAnti-Hhr6b antibodyAnti-Ubiquitin Carrier Protein B antibodyAnti-Ubiquitin-Conjugating Enzyme E2-17 Kda antibodyAnti-Ubiquitin-Protein Ligase B antibodyAnti-UBE2B antibodyAnti-RAD6B antibodyAnti-UBCH1 antibody |
Information sourced from Uniprot.org