• Immunofluorescence analysis of NIH/3T3 cells using UBE2L6 antibody (STJ115626) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using UBE2L6 antibody (STJ115626) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using UBE2L6 antibody (STJ115626) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-UBE2L6 antibody (100-153) (STJ115626)

SKU:
STJ115626

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBE2L6 (100-153) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2L6
Gene ID: 9246
Uniprot ID: UB2L6_HUMAN
Immunogen Region: 100-153
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-153 of human UBE2L6 (NP_004214.1).
Immunogen Sequence: KTCQVLEALNVLVNRPNIRE PLRMDLADLLTQNPELFRKN AEEFTLRFGVDRPS
Tissue Specificity Present in natural killer cells (at protein level).
Post Translational Modifications ISGylated.
Function Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3.
Protein Name Ubiquitin/Isg15-Conjugating Enzyme E2 L6
E2 Ubiquitin-Conjugating Enzyme L6
Retinoic Acid-Induced Gene B Protein
Rig-B
Ubch8
Ubiquitin Carrier Protein L6
Ubiquitin-Protein Ligase L6
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-168928
Reactome: R-HSA-5656169
Reactome: R-HSA-936440
Reactome: R-HSA-977225
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin/Isg15-Conjugating Enzyme E2 L6 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme L6 antibody
Anti-Retinoic Acid-Induced Gene B Protein antibody
Anti-Rig-B antibody
Anti-Ubch8 antibody
Anti-Ubiquitin Carrier Protein L6 antibody
Anti-Ubiquitin-Protein Ligase L6 antibody
Anti-UBE2L6 antibody
Anti-UBCH8 antibody

Information sourced from Uniprot.org

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