| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-UBE2L3 (73-212) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UBE2L3 |
| Gene ID: |
7332 |
| Uniprot ID: |
UB2L3_HUMAN |
| Immunogen Region: |
73-212 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 73-212 of human UBE2L3 (NP_001243284.1). |
| Immunogen Sequence: |
RKCGMKNFRNIQVDEANLLT WQGLIVPDNPPYDKGAFRIE INFPAEYPFKPPKITFKTKI YHPNIDEKGQVCLPVISAEN WKPATKTDQVIQSLIALVND PQPEHPLRADLAEEYSKDRK KFCKNAEEFTKKYGEKRPVD |
| Tissue Specificity | Ubiquitous, with highest expression in testis. |
| Post Translational Modifications | Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. Autoubiquitinated in vitro. |
| Function | Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 L3E2 Ubiquitin-Conjugating Enzyme L3L-UbcUbch7Ubiquitin Carrier Protein L3Ubiquitin-Conjugating Enzyme E2-F1Ubiquitin-Protein Ligase L3 |
| Database Links | Reactome: R-HSA-5357905Reactome: R-HSA-5675482Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 L3 antibodyAnti-E2 Ubiquitin-Conjugating Enzyme L3 antibodyAnti-L-Ubc antibodyAnti-Ubch7 antibodyAnti-Ubiquitin Carrier Protein L3 antibodyAnti-Ubiquitin-Conjugating Enzyme E2-F1 antibodyAnti-Ubiquitin-Protein Ligase L3 antibodyAnti-UBE2L3 antibodyAnti-UBCE7 antibodyAnti-UBCH7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PDCD10 knockout (KO) HeLa cells, using [KO Validated] PDCD10 Rabbit polyclonal antibody (STJ117595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/96554/379107/STJ117595_1__37100.1713146827.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PDCD10 knockout (KO) HeLa cells, using [KO Validated] PDCD10 Rabbit polyclonal antibody (STJ117595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.")
