• Biotinylated STJ70294 (0. 5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-UBE2I, Biotinylated antibody (N-Term) {Biotin} (STJ73262)

SKU:
STJ73262-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC/ICC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-UBE2I, Biotinylated (N-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: UBE2I
Gene ID: 7329
Uniprot ID: UBC9_HUMAN
Immunogen Region: N-Term
Accession Number: NP_003336.1
Specificity: Reported variants represent identical protein: NP_919237.1, NP_003336.1, NP_919236.1, NP_919235.1
Immunogen Sequence: SGIALSRLAQERKC.
Post Translational Modifications Phosphorylation at Ser-71 significantly enhances SUMOylation activity.
Function Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.
Protein Name Sumo-Conjugating Enzyme Ubc9
Ring-Type E3 Sumo Transferase Ubc9
Sumo-Protein Ligase
Ubiquitin Carrier Protein 9
Ubiquitin Carrier Protein I
Ubiquitin-Conjugating Enzyme E2 I
Ubiquitin-Protein Ligase I
P18
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-196791
Reactome: R-HSA-3065678
Reactome: R-HSA-3108214
Reactome: R-HSA-3232118
Reactome: R-HSA-3232142
Reactome: R-HSA-3899300
Reactome: R-HSA-4085377
Reactome: R-HSA-4090294
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-4655427
Reactome: R-HSA-4755510
Reactome: R-HSA-5693565
Reactome: R-HSA-5693607
Reactome: R-HSA-5696395
Reactome: R-HSA-8866904
Reactome: R-HSA-9615933
Reactome: R-HSA-9683610
Reactome: R-HSA-9694631
Reactome: R-HSA-9735871
Reactome: R-HSA-9793242
Cellular Localisation Nucleus
Cytoplasm
Perinuclear Region
Mainly Nuclear
In Spermatocytes
Localizes In Synaptonemal Complexes
Recruited By Bcl11a Into The Nuclear Body
Alternative Antibody Names Anti-Sumo-Conjugating Enzyme Ubc9 antibody
Anti-Ring-Type E3 Sumo Transferase Ubc9 antibody
Anti-Sumo-Protein Ligase antibody
Anti-Ubiquitin Carrier Protein 9 antibody
Anti-Ubiquitin Carrier Protein I antibody
Anti-Ubiquitin-Conjugating Enzyme E2 I antibody
Anti-Ubiquitin-Protein Ligase I antibody
Anti-P18 antibody
Anti-UBE2I antibody
Anti-UBC9 antibody
Anti-UBCE9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance