• Immunofluorescence analysis of NIH-3T3 cells using UBE2F antibody (STJ27140) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using UBE2F Rabbit polyclonal antibody (STJ27140) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using UBE2F Rabbit polyclonal antibody (STJ27140) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using UBE2F Rabbit polyclonal antibody (STJ27140) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using UBE2F antibody (STJ27140) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2F antibody (1-185) (STJ27140)

SKU:
STJ27140

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2F
Gene ID: 140739
Uniprot ID: UBE2F_HUMAN
Immunogen Region: 1-185
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human UBE2F (NP_542409.1).
Immunogen Sequence: MLTLASKLKRDDGLKGSRTA ATASDSTRRVSVRDKLLVKE VAELEANLPCTCKVHFPDPN KLHCFQLTVTPDEGYYQGGK FQFETEVPDAYNMVPPKVKC LTKIWHPNITETGEICLSLL REHSIDGTGWAPTRTLKDVV WGLNSLFTDLLNFDDPLNIE AAEHHLRDKEDFRNKVDDYI KRYAR
Tissue Specificity Widely expressed (at protein level).
Post Translational Modifications The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
Function Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B. Mediates neddylation of the CUL9-RBX1 complex. (Microbial infection) Following infection by HIV-1 virus, participates to HIV-1 Vif protein-mediated ubiquitination and degradation of APOBEC3G by mediating neddylation of cullin-5 (CUL5).
Protein Name Nedd8-Conjugating Enzyme Ube2f
Nedd8 Carrier Protein Ube2f
Nedd8 Protein Ligase Ube2f
Nedd8-Conjugating Enzyme 2
Ring-Type E3 Nedd8 Transferase Ube2f
Ubiquitin-Conjugating Enzyme E2 F
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Nedd8-Conjugating Enzyme Ube2f antibody
Anti-Nedd8 Carrier Protein Ube2f antibody
Anti-Nedd8 Protein Ligase Ube2f antibody
Anti-Nedd8-Conjugating Enzyme 2 antibody
Anti-Ring-Type E3 Nedd8 Transferase Ube2f antibody
Anti-Ubiquitin-Conjugating Enzyme E2 F antibody
Anti-UBE2F antibody
Anti-NCE2 antibody

Information sourced from Uniprot.org

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