Anti-Recombinant-UBE2F antibody [RM2S60] (STJA0026618)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Nedd8-Conjugating Enzyme Ube2f for use in IF, IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2S60 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200, IHC: 1:50-1:100, WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | UBE2F |
| Gene ID | 140739 |
| Uniprot ID | UBE2F_HUMAN |
Additional Info
| Post Translational Modifications | The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins. |
| Function | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B. Mediates neddylation of the CUL9-RBX1 complex. (Microbial infection) Following infection by HIV-1 virus, participates to HIV-1 Vif protein-mediated ubiquitination and degradation of APOBEC3G by mediating neddylation of cullin-5 (CUL5). |
| Protein Name | Nedd8-Conjugating Enzyme Ube2fNedd8 Carrier Protein Ube2fNedd8 Protein Ligase Ube2fNedd8-Conjugating Enzyme 2Ring-Type E3 Nedd8 Transferase Ube2fUbiquitin-Conjugating Enzyme E2 F |
| Database Links | Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nedd8-Conjugating Enzyme Ube2f antibodyAnti-Nedd8 Carrier Protein Ube2f antibodyAnti-Nedd8 Protein Ligase Ube2f antibodyAnti-Nedd8-Conjugating Enzyme 2 antibodyAnti-Ring-Type E3 Nedd8 Transferase Ube2f antibodyAnti-Ubiquitin-Conjugating Enzyme E2 F antibodyAnti-UBE2F antibodyAnti-NCE2 antibody |
Information sourced from Uniprot.org