Anti-NSFL1C antibody (1-185) (STJ28760)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NSFL1C (1-185) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NSFL1C |
| Gene ID | 55968 |
| Uniprot ID | NSF1C_HUMAN |
| Immunogen Region | 1-185 |
| Immunogen Sequence | MAAERQEALREFVAVTGAEE DRARFFLESAGWDLQIALAS FYEDGGDEDIVTISQATPSS VSRGTAPSDNRVTSFRDLIH DQDEDEEEEEGQRFYAGGSE RSGQQIVGPPRKKSPNELVD DLFKGAKEHGAVAVERVTKS PGETSKPRPFAGGGYRLGAA PEEESAYVAGEKRQHSSQDV HVVLK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2). |
Additional Info
| Post Translational Modifications | Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis. |
| Function | Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase. Also, regulates spindle orientation during mitosis. |
| Protein Name | Nsfl1 Cofactor P47Ubx Domain-Containing Protein 2cP97 Cofactor P47 |
| Database Links | Reactome: R-HSA-9013407 |
| Cellular Localisation | NucleusGolgi ApparatusGolgi StackChromosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomePredominantly Nuclear In Interphase CellsBound To The Axial Elements Of Sex Chromosomes In Pachytene SpermatocytesA Small Proportion Of The Protein Is CytoplasmicAssociated With Golgi StacksLocalizes To Centrosome During Mitotic Prophase And Metaphase |
| Alternative Antibody Names | Anti-Nsfl1 Cofactor P47 antibodyAnti-Ubx Domain-Containing Protein 2c antibodyAnti-P97 Cofactor P47 antibodyAnti-NSFL1C antibodyAnti-UBXN2C antibody |
Information sourced from Uniprot.org