• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g U2AF2/U2AF65 antibody (STJ11102100). Western blot was performed from the immunoprecipitate using U2AF2/U2AF65 antibody (STJ11102100) at a dilution of 1:1000.
  • Immunohistochemistry analysis of U2AF2/U2AF65 in paraffin-embedded human colon using U2AF2/U2AF2/U2AF65 Rabbit monoclonal antibody (STJ11102100) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using U2AF2/U2AF2/U2AF65 Rabbit monoclonal antibody (STJ11102100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using U2AF2/U2AF2/U2AF65 Rabbit monoclonal antibody (STJ11102100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-U2AF2 antibody (376-475) [S0MR] (STJ11102100)

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STJ11102100

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-U2AF65 (376-475) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: U2AF2
Gene ID: 11338
Uniprot ID: U2AF2_HUMAN
Immunogen Region: 376-475
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 376-475 of human U2AF2/U2AF2/U2AF65 (P26368).
Immunogen Sequence: EVLCLMNMVLPEELLDDEEY EEIVEDVRDECSKYGLVKSI EIPRPVDGVEVPGCGKIFVE FTSVFDCQKAMQGLTGRKFA NRVVVTKYCDPDSYHRRDFW
Post Translational Modifications Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.
Function Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals.
Protein Name Splicing Factor U2af 65 Kda Subunit
U2 Auxiliary Factor 65 Kda Subunit
Hu2af(65
Hu2af65
U2 Snrnp Auxiliary Factor Large Subunit
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72163
Reactome: R-HSA-72187
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor U2af 65 Kda Subunit antibody
Anti-U2 Auxiliary Factor 65 Kda Subunit antibody
Anti-Hu2af(65 antibody
Anti-Hu2af65 antibody
Anti-U2 Snrnp Auxiliary Factor Large Subunit antibody
Anti-U2AF2 antibody
Anti-U2AF65 antibody

Information sourced from Uniprot.org

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