Anti-U2AF1L4 antibody (Internal) (STJ71363)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-U2AF1L4 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 20kDa band observed in Human Brain (Cerebellum) and Lung lysates (calculated MW of 21.3kDa according to NP_001035515.1). Recommended concentration: 0.1-0.3ยตg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | U2AF1L4 |
| Gene ID | 199746 |
| Uniprot ID | U2AF4_HUMAN |
| Accession Number | NP_001035515.1 ; NP_659424.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | EDGERAVAELSNR |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_001035515.1 and NP_659424.2). No cross-reactivity with U2AF1 expected. |
Additional Info
| Function | RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T-cell activation.an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site. Shows a preference for AGC or AGA. |
| Protein Name | Splicing Factor U2af 26 Kda SubunitU2 Auxiliary Factor 26U2 Small Nuclear Rna Auxiliary Factor 1-Like Protein 4U2af1-Like 4U2(Rnu2 Small Nuclear Rna Auxiliary Factor 1-Like Protein 3U2 Small Nuclear Rna Auxiliary Factor 1-Like Protein 3U2af1-Like Protein 3 |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmInteraction With C1qbp Is Required For The Nuclear TranslocationDisplays Active Nucleo-Cytoplasmic Shuttling |
| Alternative Antibody Names | Anti-Splicing Factor U2af 26 Kda Subunit antibodyAnti-U2 Auxiliary Factor 26 antibodyAnti-U2 Small Nuclear Rna Auxiliary Factor 1-Like Protein 4 antibodyAnti-U2af1-Like 4 antibodyAnti-U2(Rnu2 Small Nuclear Rna Auxiliary Factor 1-Like Protein 3 antibodyAnti-U2 Small Nuclear Rna Auxiliary Factor 1-Like Protein 3 antibodyAnti-U2af1-Like Protein 3 antibodyAnti-U2AF1L4 antibodyAnti-U2AF1-RS3 antibodyAnti-U2AF1L3 antibody |
Information sourced from Uniprot.org