Anti-Tyrosine Hydroxylase antibody (31-47) (STJA0040951)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47).
General Information
| Short Description | Rabbit polyclonal anti-Tyrosine Hydroxylase (31-47) for use in IHC-F and WB in Guinea Pig, Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F/WB |
| Host | Rabbit |
| Reactivity | Guinea Pig/Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | WB 5-15 µg/mLIHC 4-10 µg/mL |
| Formulation | Lyophilized from PBS, pH 7.4, without preservatives. |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20°C for a higher stability, and at 2-8°C with an appropriate antibacteri |
Target Information
| Gene Symbol | Th |
| Gene ID | 25085 |
| Uniprot ID | TY3H_RAT |
| Immunogen | A synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47). |
| Immunogen Region | 31-47 |
| Immunogen Sequence | Human |
| Specificity | IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat, mouse and guinea pig. Cross reactivity with other species has not yet been tested. |
Additional Info
| Post Translational Modifications | Phosphorylated on Ser-19, Ser-31 and Ser-40 by several protein kinases with different site specificities. Phosphorylation at Ser-31 and Ser-40 leads to an increase of TH activity. Phosphorylation at Ser-40 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines. Phosphorylation of Ser-19 and Ser-31 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway. Phosphorylation at Ser-31 facilitates transport of TH from the soma to the nerve terminals via the microtubule network. Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG.this interaction may influence the phosphorylation and dephosphorylation of other sites. Ser-19 increases the phosphorylation at Ser-40 in a hierarchical manner, leading to increased activity. |
| Function | Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan but with lower specificity. Positively regulates the regression of retinal hyaloid vessels during postnatal development. |
| Protein Name | Tyrosine 3-MonooxygenaseTyrosine 3-HydroxylaseTh |
| Database Links | Reactome: R-RNO-209905 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleusCell ProjectionAxonCytoplasmic VesicleSecretory VesicleSynaptic VesicleWhen Phosphorylated At Ser-19 Shows A Nuclear Distribution And When Phosphorylated At Ser-31 As Well As At Ser-40 Shows A Cytosolic DistributionExpressed In Dopaminergic Axons And Axon Terminals |
| Alternative Antibody Names | Anti-Tyrosine 3-Monooxygenase antibodyAnti-Tyrosine 3-Hydroxylase antibodyAnti-Th antibodyAnti-Th antibody |
Information sourced from Uniprot.org