• Immunofluorescence analysis of paraffin-embedded Rat skin using Tyrosinase Rabbit polyclonal antibody (STJ26003) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using Tyrosinase antibody (STJ26003) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat brain, using Tyrosinase antibody (STJ26003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TYR antibody (20-340) (STJ26003)

SKU:
STJ26003

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TYR (20-340) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TYR
Gene ID: 7299
Uniprot ID: TYRO_HUMAN
Immunogen Region: 20-340
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 20-340 of human Tyrosinase (NP_000363.1).
Immunogen Sequence: PWSGDRSPCGQLSGRGSCQN ILLSNAPLGPQFPFTGVDDR ESWPSVFYNRTCQCSGNFMG FNCGNCKFGFWGPNCTERRL LVRRNIFDLSAPEKDKFFAY LTLAKHTISSDYVIPIGTYG QMKNGSTPMFNDINIYDLFV WMHYYVSMDALLGGSEIWRD IDFAHEAPAFLPWHRLFLLR WEQEIQKLTGDENFTIPYWD WRDAEKCDICTDEYMGGQHP TNPNLLSPASFFSSWQIVC
Post Translational Modifications Glycosylated.
Function This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
Protein Name Tyrosinase
Lb24-Ab
Monophenol Monooxygenase
Sk29-Ab
Tumor Rejection Antigen Ab
Database Links Reactome: R-HSA-5662702
Cellular Localisation Melanosome Membrane
Single-Pass Type I Membrane Protein
Melanosome
Proper Trafficking To Melanosome Is Regulated By Sgsm2
Ankrd27
Rab9a
Rab32 And Rab38
Alternative Antibody Names Anti-Tyrosinase antibody
Anti-Lb24-Ab antibody
Anti-Monophenol Monooxygenase antibody
Anti-Sk29-Ab antibody
Anti-Tumor Rejection Antigen Ab antibody
Anti-TYR antibody

Information sourced from Uniprot.org

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