![Western blot analysis of lysates from wild type (WT) and TYSY knockout (KO) 293T cells, using [KO Validated] TYSY Rabbit polyclonal antibody (STJ11104456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177100/368418/STJ11104456_5__76899.1713133905.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TYSY knockout (KO) 293T cells, using [KO Validated] TYSY Rabbit polyclonal antibody (STJ11104456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
Anti-TYMS antibody (220-313) (STJ11104456)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Thymidylate Synthase (220-313) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TYMS |
| Gene ID | 7298 |
| Uniprot ID | TYSY_HUMAN |
| Immunogen Region | 220-313 |
| Immunogen Sequence | GLGVPFNIASYALLTYMIAH ITGLKPGDFIHTLGDAHIYL NHIEPLKIQLQREPRPFPKL RILRKVEKIDDFKAEDFQIE GYNPHPTIKMEMAV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 220-313 of human TYSY (NP_001062.1). |
Additional Info
| Function | Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway. |
| Protein Name | Thymidylate SynthaseTsTsase |
| Database Links | Reactome: R-HSA-499943Reactome: R-HSA-69205 |
| Cellular Localisation | NucleusCytoplasmMitochondrionMitochondrion MatrixMitochondrion Inner Membrane |
| Alternative Antibody Names | Anti-Thymidylate Synthase antibodyAnti-Ts antibodyAnti-Tsase antibodyAnti-TYMS antibodyAnti-TS antibodyAnti-OK antibodyAnti-SW-cl.29 antibody |
Information sourced from Uniprot.org