• Western blot analysis of lysates from mouse kidney, using C10orf2 Rabbit polyclonal antibody (STJ115394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TWNK antibody (585-684) (STJ115394)

SKU:
STJ115394

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C10orf2 (585-684) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TWNK
Gene ID: 56652
Uniprot ID: PEO1_HUMAN
Immunogen Region: 585-684
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 585-684 of human C10orf2 (NP_068602.2).
Immunogen Sequence: NVLILQDRKLVTGPGKRYLQ VSKNRFDGDVGVFPLEFNKN SLTFSIPPKNKARLKKIKDD TGPVAKKPSSGKKGATTQNS EICSGQAPTPDQPDTSKRSK
Tissue Specificity High relative levels in skeletal muscle, testis and pancreas. Lower levels of expression in the heart, brain, placenta, lung, liver, kidney, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. Expression is coregulated with MRPL43.
Function Isoform 1: Mitochondrial helicase involved in mtDNA replication and repair. Might have a role in mtDNA repair. Has DNA strand separation activity needed to form a processive replication fork for leading strand synthesis which is catalyzed by the formation of a replisome complex with POLG and mtSDB. Preferentially unwinds DNA substrates with pre-existing 5'-and 3'- single-stranded tails but is also active on a 5'- flap substrate. Can dissociate the invading strand of immobile or mobile D-loop DNA structures irrespective of the single strand polarity of the third strand. In addition to its DNA strand separation activity, also has DNA strand annealing, DNA strand-exchange and DNA branch migration activities. Isoform 2: Lack DNA unwinding and ATP hydrolysis activities. Does not bind single-stranded or double-stranded DNA.
Protein Name Twinkle Mtdna Helicase
Progressive External Ophthalmoplegia 1 Protein
T7 Gp4-Like Protein With Intramitochondrial Nucleoid Localization
T7-Like Mitochondrial Dna Helicase
Twinkle Protein - Mitochondrial
Database Links Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion Matrix
Mitochondrion Nucleoid
Colocalizes With Mtdna In Mitochondrial Nucleoids
A Nucleoproteins Complex Consisting Of A Number Of Copies Of Proteins Associated With Mtdna
Probably Involved In Mtdna Maintenance And Expression
Alternative Antibody Names Anti-Twinkle Mtdna Helicase antibody
Anti-Progressive External Ophthalmoplegia 1 Protein antibody
Anti-T7 Gp4-Like Protein With Intramitochondrial Nucleoid Localization antibody
Anti-T7-Like Mitochondrial Dna Helicase antibody
Anti-Twinkle Protein - Mitochondrial antibody
Anti-TWNK antibody
Anti-C10orf2 antibody
Anti-PEO1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance