Anti-TWNK antibody (585-684) (STJ115394)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal C10orf2 (585-684) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TWNK |
| Gene ID | 56652 |
| Uniprot ID | PEO1_HUMAN |
| Immunogen Region | 585-684 |
| Immunogen Sequence | NVLILQDRKLVTGPGKRYLQ VSKNRFDGDVGVFPLEFNKN SLTFSIPPKNKARLKKIKDD TGPVAKKPSSGKKGATTQNS EICSGQAPTPDQPDTSKRSK |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 585-684 of human C10orf2 (NP_068602.2). |
Additional Info
| Tissue Specificity | High relative levels in skeletal muscle, testis and pancreas. Lower levels of expression in the heart, brain, placenta, lung, liver, kidney, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. Expression is coregulated with MRPL43. |
| Function | Isoform 1: Mitochondrial helicase involved in mtDNA replication and repair. Might have a role in mtDNA repair. Has DNA strand separation activity needed to form a processive replication fork for leading strand synthesis which is catalyzed by the formation of a replisome complex with POLG and mtSDB. Preferentially unwinds DNA substrates with pre-existing 5'-and 3'- single-stranded tails but is also active on a 5'- flap substrate. Can dissociate the invading strand of immobile or mobile D-loop DNA structures irrespective of the single strand polarity of the third strand. In addition to its DNA strand separation activity, also has DNA strand annealing, DNA strand-exchange and DNA branch migration activities. Isoform 2: Lack DNA unwinding and ATP hydrolysis activities. Does not bind single-stranded or double-stranded DNA. |
| Protein Name | Twinkle Mtdna HelicaseProgressive External Ophthalmoplegia 1 ProteinT7 Gp4-Like Protein With Intramitochondrial Nucleoid LocalizationT7-Like Mitochondrial Dna HelicaseTwinkle Protein - Mitochondrial |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-9837999Reactome: R-HSA-9913635 |
| Cellular Localisation | Mitochondrion MatrixMitochondrion NucleoidMitochondrion Inner MembranePeripheral Membrane ProteinColocalizes With Mtdna In Mitochondrial NucleoidsA Nucleoproteins Complex Consisting Of A Number Of Copies Of Proteins Associated With MtdnaProbably Involved In Mtdna Maintenance And ExpressionAssociates With Phospholipid Membranes Via Electrostatic BindingPreferentially Associates With Membranes Enriched With CardiolipinA Lipid Abundant In The Mitochondrial Inner MembraneAtpase And Helicase Activity Is Enhanced By Binding To Lipid Membranes |
| Alternative Antibody Names | Anti-Twinkle Mtdna Helicase antibodyAnti-Progressive External Ophthalmoplegia 1 Protein antibodyAnti-T7 Gp4-Like Protein With Intramitochondrial Nucleoid Localization antibodyAnti-T7-Like Mitochondrial Dna Helicase antibodyAnti-Twinkle Protein - Mitochondrial antibodyAnti-TWNK antibodyAnti-C10orf2 antibodyAnti-PEO1 antibody |
Information sourced from Uniprot.org