• Western blot analysis of extracts of various cell lines, using Gamma-Tubulin rabbit monoclonal antibody (STJ11102519) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Confocal imaging of U-2OS cells using Gamma-Tubulin Rabbit monoclonal antibody (STJ11102519, dilution 1:100) (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit monoclonal antibody ( dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry of paraffin-embedded rat testis using Gamma-Tubulin rabbit monoclonal antibody (STJ11102519) at dilution of 1:100 (40x lens).
  • Confocal imaging of NIH-3T3 cells using Gamma-Tubulin Rabbit monoclonal antibody (STJ11102519, dilution 1:100) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit monoclonal antibody ( dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry of paraffin-embedded human thyroid cancer using Gamma-Tubulin rabbit monoclonal antibody (STJ11102519) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Gamma-Tubulin in paraffin-embedded mouse testis using Gamma-Tubulin Rabbit monoclonal antibody (STJ11102519) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using Gamma-Tubulin rabbit monoclonal antibody (STJ11102519) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Gamma-Tubulin in paraffin-embedded human thyroid cancer using Gamma-Tubulin Rabbit monoclonal antibody (STJ11102519) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Gamma-Tubulin rabbit monoclonal antibody (STJ11102519) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Gamma-Tubulin in paraffin-embedded rat testis using Gamma-Tubulin Rabbit monoclonal antibody (STJ11102519) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Gamma-Tubulin rabbit monoclonal antibody (STJ11102519) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Gamma-Tubulin Rabbit monoclonal antibody (STJ11102519) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of U-2 OS cells using Gamma-Tubulin rabbit monoclonal antibody (STJ11102519) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TUBG1 antibody (231-451) [S9MR] (STJ11102519)

SKU:
STJ11102519

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Gamma-Tubulin (231-451) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TUBG1
Gene ID: 7283
Uniprot ID: TBG1_HUMAN
Immunogen Region: 231-451
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 231-451 of human Gamma-Tubulin (P23258).
Immunogen Sequence: LVSTIMSASTTTLRYPGYMN NDLIGLIASLIPTPRLHFLM TGYTPLTTDQSVASVRKTTV LDVMRRLLQPKNVMVSTGRD RQTNHCYIAILNIIQGEVDP TQVHKSLQRIRERKLANFIP WGPASIQVALSRKSPYLPSA HRVSGLMMANHTSISSLFER TCRQYDKLRKREAFLEQFRK EDMFKDNFDEMDTSREIVQQ LIDEYHAATRPDYISWGTQE Q
Post Translational Modifications Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Function Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
Protein Name Tubulin Gamma-1 Chain
Gamma-1-Tubulin
Gamma-Tubulin Complex Component 1
Gcp-1
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Localizes To Mitotic Spindle Microtubules
Alternative Antibody Names Anti-Tubulin Gamma-1 Chain antibody
Anti-Gamma-1-Tubulin antibody
Anti-Gamma-Tubulin Complex Component 1 antibody
Anti-Gcp-1 antibody
Anti-TUBG1 antibody
Anti-TUBG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance