• Western blot analysis of extracts of various cell lines, using Beta III-Tubulin/Beta 3-Tubulin antibody (STJ11101457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of Beta III-Tubulin in paraffin-embedded mouse brain tissue using Beta III-Tubulin rabbit monoclonal antibody (A20018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Beta III-Tubulin in paraffin-embedded rat brain tissue using Beta III-Tubulin rabbit monoclonal antibody (A20018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Confocal imaging of frozen sections of Rat brain using Beta III-Tubulin/Beta 3-Tubulin rabbit monoclonal antibody (STJ11101457, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of frozen sections of mouse brain using Beta III-Tubulin/Beta 3-Tubulin rabbit monoclonal antibody (STJ11101457, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of SH-SY5Y cells using Beta III-Tubulin rabbit monoclonal antibody (STJ11101457, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-TUBB3 antibody (351-450) [S7MR] (STJ11101457)

SKU:
STJ11101457

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Beta III-Tubulin (351-450) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TUBB3
Gene ID: 10381
Uniprot ID: TBB3_HUMAN
Immunogen Region: 351-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 351-450 of human Beta III-Tubulin (Q13509).
Immunogen Sequence: VAVCDIPPRGLKMSSTFIGN STAIQELFKRISEQFTAMFR RKAFLHWYTGEGMDEMEFTE AESNMNDLVSEYQQYQDATA EEEGEMYEDDEEESEAQGPK
Tissue Specificity Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
Post Translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord.
Protein Name Tubulin Beta-3 Chain
Tubulin Beta-4 Chain
Tubulin Beta-Iii
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-190840
Reactome: R-HSA-190861
Reactome: R-HSA-2132295
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-3371497
Reactome: R-HSA-380320
Reactome: R-HSA-389957
Reactome: R-HSA-389960
Reactome: R-HSA-389977
Reactome: R-HSA-437239
Reactome: R-HSA-5610787
Reactome: R-HSA-5617833
Reactome: R-HSA-5620924
Reactome: R-HSA-5626467
Reactome: R-HSA-5663220
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-6811436
Reactome: R-HSA-68877
Reactome: R-HSA-8852276
Reactome: R-HSA-8955332
Reactome: R-HSA-9609690
Reactome: R-HSA-9609736
Reactome: R-HSA-9619483
Reactome: R-HSA-9646399
Reactome: R-HSA-9648025
Reactome: R-HSA-9668328
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Growth Cone
Lamellipodium
Filopodium
Alternative Antibody Names Anti-Tubulin Beta-3 Chain antibody
Anti-Tubulin Beta-4 Chain antibody
Anti-Tubulin Beta-Iii antibody
Anti-TUBB3 antibody
Anti-TUBB4 antibody

Information sourced from Uniprot.org

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