• Immunofluorescence analysis of Hep G2 cells using TTR Rabbit polyclonal antibody (STJ25986) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TTR in paraffin-embedded mouse lung using TTR Rabbit polyclonal antibody (STJ25986) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TTR Rabbit polyclonal antibody (STJ25986) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of lysates from HepG2 cells, using TTR Rabbit polyclonal antibody (STJ25986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-TTR antibody (21-147) (STJ25986)

SKU:
STJ25986

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TTR (21-147) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TTR
Gene ID: 7276
Uniprot ID: TTHY_HUMAN
Immunogen Region: 21-147
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-147 of human TTR (NP_000362.1).
Immunogen Sequence: GPTGTGESKCPLMVKVLDAV RGSPAINVAVHVFRKAADDT WEPFASGKTSESGELHGLTT EEEFVEGIYKVEIDTKSYWK ALGISPFHEHAEVVFTANDS GPRRYTIAALLSPYSYSTTA VVTNPKE
Tissue Specificity Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
Post Translational Modifications Not glycosylated under normal conditions. Following unfolding, caused for example by variant AMYL-TTR 'Gly-38', the cryptic Asn-118 site is exposed and glycosylated by STT3B-containing OST complex, leading to its degradation by the ER-associated degradation (ERAD) pathway. Sulfonation of the reactive cysteine Cys-30 enhances the stability of the native conformation of TTR, avoiding misassembly of the protein leading to amyloid formation.
Function Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Protein Name Transthyretin
Attr
Prealbumin
Tbpa
Database Links Reactome: R-HSA-2453864
Reactome: R-HSA-2453902
Reactome: R-HSA-3000171
Reactome: R-HSA-6798695
Reactome: R-HSA-975634
Reactome: R-HSA-977225
Cellular Localisation Secreted
Cytoplasm
Alternative Antibody Names Anti-Transthyretin antibody
Anti-Attr antibody
Anti-Prealbumin antibody
Anti-Tbpa antibody
Anti-TTR antibody
Anti-PALB antibody

Information sourced from Uniprot.org

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