| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TTLL12 (1-450) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TTLL12 |
| Gene ID: |
23170 |
| Uniprot ID: |
TTL12_HUMAN |
| Immunogen Region: |
1-450 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-450 of human TTLL12 (NP_055955.1). |
| Immunogen Sequence: |
MEAERGPERRPAERSSPGQT PEEGAQALAEFAALHGPALR ASGVPERYWGRLLHKLEHEV FDAGEVFGIMQVEEVEEEED EAAREVRKQQPNPGNELCYK VIVTRESGLQAAHPNSIFLI DHAWTCRVEHARQQLQQVPG LLHRMANLMGIEFHGELPST EAVALVLEEMWKFNQTYQLA HGTAEEKMPVWYIMDEFGSR IQHADVPSFATAPFFYMPQQ VAYTLLWPLRDLDTGEEVT |
| Tissue Specificity | Expressed in the basal layer of prostate and endothelial cells. Increased expression in prostatic intraepithelial neoplasia and metastatic lesions. |
| Function | Negatively regulates post-translational modifications of tubulin, including detyrosination of the C-terminus and polyglutamylation of glutamate residues. Also, indirectly promotes histone H4 trimethylation at 'Lys-20' (H4K20me3). Probably by controlling tubulin and/or histone H4 post-translational modifications, plays a role in mitosis and in maintaining chromosome number stability. During RNA virus-mediated infection, acts as a negative regulator of the RIG-I pathway by preventing MAVS binding to TBK1 and IKBKE. |
| Protein Name | Tubulin--Tyrosine Ligase-Like Protein 12Inactive Tubulin--Tyrosine Ligase-Like Protein 12 |
| Database Links | Reactome: R-HSA-8955332 |
| Cellular Localisation | CytoplasmMidbodyCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleNucleusPredominantly Localizes In The CytoplasmPartially Colocalizes With Vimentin In Prostate Cancer Cells |
| Alternative Antibody Names | Anti-Tubulin--Tyrosine Ligase-Like Protein 12 antibodyAnti-Inactive Tubulin--Tyrosine Ligase-Like Protein 12 antibodyAnti-TTLL12 antibodyAnti-KIAA0153 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TTLL12 knockout (KO) 293T cells, using [KO Validated] TTLL12 Rabbit polyclonal antibody (STJ11100870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89804/358271/STJ11100870_1__29815.1713122614.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TTLL12 knockout (KO) 293T cells, using [KO Validated] TTLL12 Rabbit polyclonal antibody (STJ11100870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
