Anti-TTC8 antibody (Internal) (STJ72220)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-TTC8 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Dog/Cow/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 60kDa band observed in Human Testis lysates (calculated MW of 58.4kDa according to NP_653197.2;). Recommended concentration: 0.2-0.6µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | TTC8 |
| Gene ID | 123016 |
| Uniprot ID | TTC8_HUMAN |
| Accession Number | NP_653197.2; NP_938051.1; NP_938052.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | KEVLKQDNTHVE |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_653197.2; NP_938051.1; NP_938052.1). |
Additional Info
| Function | The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. |
| Protein Name | Tetratricopeptide Repeat Protein 8Tpr Repeat Protein 8Bardet-Biedl Syndrome 8 Protein |
| Database Links | Reactome: R-HSA-5620922 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCell ProjectionCilium MembraneCentriolar SatelliteCilium |
| Alternative Antibody Names | Anti-Tetratricopeptide Repeat Protein 8 antibodyAnti-Tpr Repeat Protein 8 antibodyAnti-Bardet-Biedl Syndrome 8 Protein antibodyAnti-TTC8 antibodyAnti-BBS8 antibody |
Information sourced from Uniprot.org