Anti-TTBK2 antibody (1185-1244) (STJ29746)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TTBK2 (1185-1244) antibody for use in WB, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TTBK2 |
| Gene ID | 146057 |
| Uniprot ID | TTBK2_HUMAN |
| Immunogen Region | 1185-1244 |
| Immunogen Sequence | LGRSKSPPSHSGSSSSRRSC QQEHCKPSKNGLKGSGSLHH HSASTKTPQGKSKPASKLSR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1185-1244 of human TTBK2 (NP_775771.3). |
Additional Info
| Function | Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro. Phosphorylates MPHOSPH9 which promotes its ubiquitination and proteasomal degradation, loss of MPHOSPH9 facilitates the removal of the CP110-CEP97 complex (a negative regulator of ciliogenesis) from the mother centrioles, promoting the initiation of ciliogenesis. Required for recruitment of CPLANE2 and INTU to the mother centriole. |
| Protein Name | Tau-Tubulin Kinase 2 |
| Database Links | Reactome: R-HSA-5620912 |
| Cellular Localisation | Cell ProjectionCiliumCytoplasmCytoskeletonCilium Basal BodyMicrotubule Organizing CenterCentrosomeCentrioleCytosolNucleusLocalizes At The Transition ZoneA Region Between The Basal Body And The Ciliary AxonemeMay Also Be Present In Cytosol AndAt Lower Level In The Nucleus |
| Alternative Antibody Names | Anti-Tau-Tubulin Kinase 2 antibodyAnti-TTBK2 antibodyAnti-KIAA0847 antibody |
Information sourced from Uniprot.org