• Immunofluorescence analysis of U-2 OS cells using TSLP Rabbit polyclonal antibody (STJ115100) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using TSLP Rabbit polyclonal antibody (STJ115100) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using TSLP Rabbit polyclonal antibody (STJ115100) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using TSLP Rabbit polyclonal antibody (STJ115100) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TSLP antibody (29-159) (STJ115100)

SKU:
STJ115100

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TSLP (29-159) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSLP
Gene ID: 85480
Uniprot ID: TSLP_HUMAN
Immunogen Region: 29-159
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-159 of human TSLP (NP_149024.1).
Immunogen Sequence: YDFTNCDFEKIKAAYLSTIS KDLITYMSGTKSTEFNNTVS CSNRPHCLTEIQSLTFNPTA GCASLAKEMFAMKTKAALAI WCPGYSETQINATQAMKKRR KRKVTTNKCLEQVSQLQGLW RRFNRPLLKQQ
Tissue Specificity Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva.
Function Isoform 1: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. Isoform 2: May act as an antimicrobial peptide in the oral cavity and on the skin.
Protein Name Thymic Stromal Lymphopoietin
Database Links Reactome: R-HSA-1266695
Cellular Localisation Secreted
Alternative Antibody Names Anti-Thymic Stromal Lymphopoietin antibody
Anti-TSLP antibody

Information sourced from Uniprot.org

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