• Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using TSHR Rabbit polyclonal antibody (STJ28864) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat kidney, using TSHR antibody (STJ28864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TSHR antibody (21-253) (STJ28864)

SKU:
STJ28864

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TSHR (21-253) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSHR
Gene ID: 7253
Uniprot ID: TSHR_HUMAN
Immunogen Region: 21-253
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-253 of human TSHR (NP_001018046.1).
Immunogen Sequence: GMGCSSPPCECHQEEDFRVT CKDIQRIPSLPPSTQTLKLI ETHLRTIPSHAFSNLPNISR IYVSIDVTLQQLESHSFYNL SKVTHIEIRNTRNLTYIDPD ALKELPLLKFLGIFNTGLKM FPDLTKVYSTDIFFILEITD NPYMTSIPVNAFQGLCNETL TLKLYNNGFTSVQGYAFNGT KLDAVYLNKNKYLTVIDKDA FGGVYSGPSLLLPLGRKSLS FETQKAPRSSMPS
Tissue Specificity Expressed in thyroide cells (at protein level). Expressed in the thyroid.
Post Translational Modifications Glycosylated. Sulfated. Sulfation on Tyr-385 plays a role in thyrotropin receptor binding and activation.
Function Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism.
Protein Name Thyrotropin Receptor
Thyroid-Stimulating Hormone Receptor
Tsh-R
Database Links Reactome: R-HSA-375281
Reactome: R-HSA-418555
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Alternative Antibody Names Anti-Thyrotropin Receptor antibody
Anti-Thyroid-Stimulating Hormone Receptor antibody
Anti-Tsh-R antibody
Anti-TSHR antibody
Anti-LGR3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance