Anti-TSG101 antibody [PT0240R] (STJA0039663)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgGk
STJA0039663
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General Information

Short DescriptionRabbit monoclonal anti-Tumor Susceptibility Gene 101 Protein for use in WB, IF, IP and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/IP/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDPT0240R
IsotypeIgGk
ConjugationUnconjugated
PurificationProtein A
Dilution RangeWB 1:1000-1:5000
IF 1:200-1:1000
ELISA 1:5000-1:20000
IP 1:50-1:200
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTSG101
Gene ID7251
Uniprot IDTS101_HUMAN
SpecificityEndogenous

Additional Info

Post Translational Modifications Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis.the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-ST-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan. It may also play a role in the extracellular release of microvesicles that differ from the exosomes.
Protein Name Tumor Susceptibility Gene 101 Protein
Escrt-I Complex Subunit Tsg101
Database Links Reactome: R-HSA-162588
Reactome: R-HSA-174490
Reactome: R-HSA-917729
Reactome: R-HSA-9610379
Reactome: R-HSA-9615710
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Late Endosome Membrane
Cytoskeleton
Microtubule Organizing Center
Centrosome
Midbody
Midbody Ring
Nucleus
Mainly Cytoplasmic
Membrane-Associated When Active And Soluble When Inactive
Nuclear Localization Is Cell Cycle-Dependent
Interaction With Cep55 Is Required For Localization To The Midbody During Cytokinesis
Alternative Antibody Names Anti-Tumor Susceptibility Gene 101 Protein antibody
Anti-Escrt-I Complex Subunit Tsg101 antibody
Anti-TSG101 antibody

Information sourced from Uniprot.org

Citations

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