• Immunofluorescence analysis of PC-12 cells using TSG101/VPS23 Rabbit polyclonal antibody (STJ11104735) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using TSG101/VPS23 Rabbit polyclonal antibody (STJ11104735) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from normal (control) and TSG101/VPS23 knockdown (KD) SH-SY5Y (KD) cells, using TSG101/VPS23 Rabbit polyclonal antibody (STJ11104735) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of K-562, using TSG101/VPS23 Rabbit polyclonal antibody (STJ11104735) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TSG101 antibody (291-390) (STJ11104735)

SKU:
STJ11104735

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tumor Susceptibility Gene 101 Protein (291-390) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSG101
Gene ID: 7251
Uniprot ID: TS101_HUMAN
Immunogen Region: 291-390
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 291-390 of human TSG101/VPS23 (NP_006283.1).
Immunogen Sequence: LKKKDEELSSALEKMENQSE NNDIDEVIIPTAPLYKQILN LYAEENAIEDTIFYLGEALR RGVIDLDVFLKHVRLLSRKQ FQLRALMQKARKTAGLSDLY
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
Post Translational Modifications Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis.the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-ST-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan. It may also play a role in the extracellular release of microvesicles that differ from the exosomes.
Protein Name Tumor Susceptibility Gene 101 Protein
Escrt-I Complex Subunit Tsg101
Database Links Reactome: R-HSA-162588
Reactome: R-HSA-174490
Reactome: R-HSA-917729
Reactome: R-HSA-9610379
Reactome: R-HSA-9615710
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Late Endosome Membrane
Cytoskeleton
Microtubule Organizing Center
Centrosome
Midbody
Midbody Ring
Nucleus
Mainly Cytoplasmic
Membrane-Associated When Active And Soluble When Inactive
Nuclear Localization Is Cell Cycle-Dependent
Interaction With Cep55 Is Required For Localization To The Midbody During Cytokinesis
Alternative Antibody Names Anti-Tumor Susceptibility Gene 101 Protein antibody
Anti-Escrt-I Complex Subunit Tsg101 antibody
Anti-TSG101 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance