Anti-TRPV1 antibody (4th Cytoplasmic Loop) (STJ13100491)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the 4th cytoplasmic loop of rat TRPV1 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-TRPV1 (4th Cytoplasmic Loop) for use in IHC and WB in Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:1000 to 1:2000 is recommended for WB and 1:200 to 1:500 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Trpv1 |
| Gene ID | 83810 |
| Uniprot ID | TRPV1_RAT |
| Immunogen | A synthetic peptide from the 4th cytoplasmic loop of rat TRPV1 conjugated to blue carrier protein was used as the antigen. |
| Immunogen Region | 4th Cytoplasmic Loop |
| Specificity | Specific for TRPV1. |
Additional Info
| Post Translational Modifications | Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-116 as a major phosphorylation site. Phosphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PRKCE, PRKCM and probably PRKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. |
| Function | Non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius. Upon activation, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis. Isoform 3: Does not display channel activity in response to noxious chemical compounds, such as capsaicin and the vanilloid resiniferatoxin. Channel activity is not elicited by mildly acidic extracellular pH, and only slight channel activity is observed in response to noxiuos heat stimuli. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily V Member 1Trpv1Capsaicin ReceptorOsm-9-Like Trp Channel 1Otrpc1Vanilloid Receptor 1Vanilloid Receptor Type 1-Like |
| Database Links | Reactome: R-RNO-3295583 |
| Cellular Localisation | Postsynaptic Cell MembraneMulti-Pass Membrane ProteinCell ProjectionDendritic Spine MembraneCell MembraneMostlyBut Not Exclusively Expressed In Postsynaptic Dendritic Spines |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily V Member 1 antibodyAnti-Trpv1 antibodyAnti-Capsaicin Receptor antibodyAnti-Osm-9-Like Trp Channel 1 antibodyAnti-Otrpc1 antibodyAnti-Vanilloid Receptor 1 antibodyAnti-Vanilloid Receptor Type 1-Like antibodyAnti-Trpv1 antibodyAnti-Vr1 antibodyAnti-Vr1l antibody |
Information sourced from Uniprot.org