| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRPM2 (4th Cytoplasmic Domain) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
TRPM2 |
| Gene ID: |
7226 |
| Uniprot ID: |
TRPM2_HUMAN |
| Immunogen Region: |
4th Cytoplasmic Domain |
| Specificity: |
Specific for TRPM2. |
| Immunogen: |
A synthetic peptide from the 4th cytoplasmic domain of human TRPM2 (TRPC7, LTRPC2) conjugated to an immunogenic carrier protein was used as the antigen. |
| Post Translational Modifications | Phosphorylation of TRPM2 at Thr-740 by protein kinase C (PKC) counteracts the effect of cytosolic Ca(2+) and elevates the temperature threshold. |
| Function | Isoform 1: Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels. Functions as a ligand-gated ion channel, gated by intracellular adenosine diphosphate ribose (ADP-ribose), Ca(2+), warm temperature, and oxidative stress. The precise physiological activators are under debate.the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change.the channel is primed but still requires Ca(2+) binding to trigger channel opening. Extracellular Ca(2+) passes through the channel and increases channel activity. Contributes to Ca(2+) release from intracellular stores in response to ADP-ribose. Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (Probable). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels. Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels. Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels. Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its role in increasing cytoplasmic Ca(2+) and Zn(2+) levels. Confers susceptibility to cell death following oxidative stress. Isoform 2: Lacks cation channel activity. Does not mediate cation transport in response to oxidative stress or ADP-ribose. Isoform 3: Lacks cation channel activity and negatively regulates the channel activity of isoform 1. Negatively regulates susceptibility to cell death in reposponse to oxidative stress. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 2Estrogen-Responsive Element-Associated Gene 1 ProteinLong Transient Receptor Potential Channel 2Ltrpc-2Ltrpc2Transient Receptor Potential Channel 7Trpc7Transient Receptor Potential Melastatin 2 |
| Database Links | Reactome: R-HSA-3295583Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinPerikaryonCell ProjectionCytoplasmic VesicleLysosomeDetected At The Cell Membrane And In Intracellular Vesicles In Cortical NeuronsDetected On Neuronal Cell Bodies And NeuritesDetected On The Cell Membrane In Polymorphonuclear NeutrophilsDetected On Cytoplasmic Vesicles And Lysosomes In Immature Bone Marrow Dendritic CellsIsoform 1: Cell MembraneIsoform 2: Cell MembraneIsoform 3: Cell Membrane |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily M Member 2 antibodyAnti-Estrogen-Responsive Element-Associated Gene 1 Protein antibodyAnti-Long Transient Receptor Potential Channel 2 antibodyAnti-Ltrpc-2 antibodyAnti-Ltrpc2 antibodyAnti-Transient Receptor Potential Channel 7 antibodyAnti-Trpc7 antibodyAnti-Transient Receptor Potential Melastatin 2 antibodyAnti-TRPM2 antibodyAnti-EREG1 antibodyAnti-KNP3 antibodyAnti-LTRPC2 antibodyAnti-TRPC7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
