• STJ73487 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ73487 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ73487 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-TRPC1 antibody (Internal) (STJ73487)

SKU:
STJ73487-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC/IHC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-TRPC1 (Internal) is suitable for use in ELISA, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
IF-Strong expression of the protein seen in the cytoplasm/vesicles of HeLa cells. Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TRPC1
Gene ID: 7220
Uniprot ID: TRPC1_HUMAN
Immunogen Region: Internal
Accession Number: NP_003295.1; NP_001238774.1
Immunogen Sequence: NLKQKRDENYQK
Post Translational Modifications Activation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells.
Function Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.
Protein Name Short Transient Receptor Potential Channel 1
Trpc1
Transient Receptor Protein 1
Trp-1
Database Links Reactome: R-HSA-3295583
Reactome: R-HSA-418890
Reactome: R-HSA-5578775
Reactome: R-HSA-983695
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Short Transient Receptor Potential Channel 1 antibody
Anti-Trpc1 antibody
Anti-Transient Receptor Protein 1 antibody
Anti-Trp-1 antibody
Anti-TRPC1 antibody
Anti-TRP1 antibody

Information sourced from Uniprot.org

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