• Immunofluorescence analysis of human liver cancer cells using KIAA1456 Rabbit polyclonal antibody (STJ11105387) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of KIAA1456 in paraffin-embedded Rat brain tissue using KIAA1456 Rabbit polyclonal antibody (STJ11105387) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KIAA1456 in paraffin-embedded Mouse intestin tissue using KIAA1456 Rabbit polyclonal antibody (STJ11105387) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KIAA1456 in paraffin-embedded Human cervix tissue using KIAA1456 Rabbit polyclonal antibody (STJ11105387) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using KIAA1456 Rabbit polyclonal antibody (STJ11105387) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : THP-1. Exposure time: 5s.

Anti-TRMT9B antibody (150-250) (STJ11105387)

SKU:
STJ11105387

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Probable Trna Methyltransferase 9b (150-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRMT9B
Gene ID: 57604
Uniprot ID: TRM9B_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human KIAA1456 (NP_065895.2).
Immunogen Sequence: VWAMEQKNRHFEKQDVLVPW NRALCSQLFSESSQSGRKRQ CGYPERGHPYHPPCSECSCS VCFKEQCGSKRSHSVGYEPA MARTCFANISKEGEEEYGFY STLGKSFRSW
Tissue Specificity Down-regulated in breast, bladder, colorectal, cervix and testicular carcinomas.
Function May modify wobble uridines in specific arginine and glutamic acid tRNAs. Acts as a tumor suppressor by promoting the expression of LIN9.
Protein Name Probable Trna Methyltransferase 9b
Probable Trna Methyltransferase 9-Like Protein
Database Links Reactome: R-HSA-6782315
Alternative Antibody Names Anti-Probable Trna Methyltransferase 9b antibody
Anti-Probable Trna Methyltransferase 9-Like Protein antibody
Anti-TRMT9B antibody
Anti-C8orf79 antibody
Anti-KIAA1456 antibody
Anti-TRM9L antibody

Information sourced from Uniprot.org

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