• Western blot analysis of extracts of mouse liver, using TRMT61B antibody (STJ110370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using TRMT61B antibody (STJ110370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TRMT61B antibody (1-250) (STJ110370)

SKU:
STJ110370

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRMT61B (1-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRMT61B
Gene ID: 55006
Uniprot ID: TR61B_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT61B (NP_060380.3).
Immunogen Sequence: MLMAWCRGPVLLCLRQGLGT NSFLHGLGQEPFEGARSLCC RSSPRDLRDGEREHEAAQRK APGAESCPSLPLSISDIGTG CLSSLENLRLPTLREESSPR ELEDSSGDQGRCGPTHQGSE DPSMLSQAQSATEVEERHVS PSCSTSRERPFQAGELILAE TGEGETKFKKLFRLNNFGLL NSNWGAVPFGKIVGKFPGQI LRSSFGKQYMLRRPALEDYV VLMKRGTAITFPKDINMIL
Function Methyltransferase that catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in various tRNAs in mitochondrion, including tRNA(Leu) (deciphering codons UUA or UUG), tRNA(Lys) and tRNA(Ser) (deciphering codons UCA, UCU, UCG or UCC). Catalyzes the formation of 1-methyladenosine at position 947 of mitochondrial 16S ribosomal RNA and this modification is most likely important for mitoribosomal structure and function. In addition to tRNA N(1)-methyltransferase activity, also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA, leading to interfere with mitochondrial translation.
Protein Name Trna
Adenine(58-N(1-Methyltransferase - Mitochondrial
Mrna Methyladenosine-N(1-Methyltransferase
Database Links Reactome: R-HSA-6787450
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Trna antibody
Anti-Adenine(58-N(1-Methyltransferase - Mitochondrial antibody
Anti-Mrna Methyladenosine-N(1-Methyltransferase antibody
Anti-TRMT61B antibody

Information sourced from Uniprot.org

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