Anti-TRIOBP antibody (STJA0007402)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human TRIOBP
General Information
| Short Description | Rabbit polyclonal anti-TRIO and F-actin-binding protein for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIOBP |
| Gene ID | 11078 |
| Uniprot ID | TARA_HUMAN |
| Immunogen | Synthesized peptide derived from human TRIOBP |
| Specificity | This antibody detects endogenous levels of TRIOBP at Human, Mouse |
Additional Info
| Post Translational Modifications | Isoform 1: Ubiquitinated by HECTD3, leading to its degradation by the proteasome. Isoform 1: Phosphorylation at Thr-457 by PLK1 ensures mitotic progression and is essential for accurate chromosome segregation. Phosphorylation at residues Thr-221 and Thr-457 by kinase NEK2A and PLK1 coordinates TERF1 translocation from telomere to spindle pole. |
| Function | Isoform 1: Regulates actin cytoskeletal organization, cell spreading and cell contraction by directly binding and stabilizing filamentous F-actin and prevents its depolymerization. May also serve as a linker protein to recruit proteins required for F-actin formation and turnover. Essential for correct mitotic progression. Isoform 5: Plays a pivotal role in the formation of stereocilia rootlets. Isoform 4: Plays a pivotal role in the formation of stereocilia rootlets. |
| Protein Name | Trio And F-Actin-Binding ProteinProtein TaraTrf1-Associated Protein Of 68 KdaTrio-Associated Repeat On Actin |
| Database Links | Reactome: R-HSA-9662360Reactome: R-HSA-9662361 |
| Cellular Localisation | NucleusIsoform 1: NucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeMidbodyChromosomeTelomereCentrosomal Localization Occurs Upon Phosphorylation By Plk1 At Thr-457 And Lasts From Prophase To AnaphaseAt TelophaseRelocalizes To Midbody |
| Alternative Antibody Names | Anti-Trio And F-Actin-Binding Protein antibodyAnti-Protein Tara antibodyAnti-Trf1-Associated Protein Of 68 Kda antibodyAnti-Trio-Associated Repeat On Actin antibodyAnti-TRIOBP antibodyAnti-KIAA1662 antibodyAnti-TARA antibodyAnti-HRIHFB2122 antibody |
Information sourced from Uniprot.org