• Western blot analysis of various lysates, using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K36 antibody (STJ23992).
  • Immunohistochemistry analysis of paraffin-embedded human lung using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human spleen using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K36 antibody (STJ23992). Blue: DAPI for nuclear staining.
  • Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using TriMethyl-Histone H3-K36 rabbit polyclonal antibody antibody (STJ23992) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K36 antibody (STJ23992) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Chromatin immunoprecipitation analysis of extracts of Hale cells, using TriMethyl-Histone H3-K36 antibody (STJ23992) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Tri-Methyl-Histone H3-K36 antibody (32-42) (STJ23992)

SKU:
STJ23992

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tri-Methyl-Histone H3-K36 (32-42) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:20-1:100
ChIP-seq 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 32-42
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 32-42 of human histone H3 (NP_003520.1).
Immunogen Sequence: ATGGVKKPHRY
Background This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

Information sourced from Uniprot.org

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