Anti-TRIM72 antibody (Internal) (STJ72398)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-TRIM72 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 52kDa band observed in Mouse Heart lysates (calculated MW of 52.8kDa according to Mouse NP_001073401.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM72 |
| Gene ID | 493829 |
| Uniprot ID | TRI72_HUMAN |
| Accession Number | NP_001008275.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | RPQALSTNLQ |
Additional Info
| Function | Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. |
| Protein Name | Tripartite Motif-Containing Protein 72Mitsugumin-53Mg53 |
| Database Links | Reactome: R-HSA-445355 |
| Cellular Localisation | Cell MembraneSarcolemmaCytoplasmic Vesicle MembraneTethered To Plasma Membrane And Cytoplasmic Vesicles Via Its Interaction With Phosphatidylserine |
| Alternative Antibody Names | Anti-Tripartite Motif-Containing Protein 72 antibodyAnti-Mitsugumin-53 antibodyAnti-Mg53 antibodyAnti-TRIM72 antibodyAnti-MG53 antibody |
Information sourced from Uniprot.org