Anti-TRIM72 antibody (200-300 aa) (STJ11106230)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TRIM72 (200-300 aa) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Trim72 |
| Gene ID | 434246 |
| Uniprot ID | TRI72_MOUSE |
| Immunogen Region | 200-300 aa |
| Immunogen Sequence | DAGVALRRELSSLNSYLEQL RQMEKVLEEVADKPQTEFLM KFCLVTSRLQKILSESPPPA RLDIQLPVISDDFKFQVWKK MFRALMPALEELTFDPSSAH P |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 200-300 of mouse TRIM72 (NP_001073401.1). |
Additional Info
| Tissue Specificity | Muscle-specific. Exclusively expressed in cardiac and skeletal muscle. |
| Post Translational Modifications | Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization. S-nitrosylation at Cys-144 stabilizes TRIM72 and protects against oxidation-induced protein degradation and cell death. |
| Function | Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. |
| Protein Name | Tripartite Motif-Containing Protein 72Mitsugumin-53Mg53 |
| Database Links | |
| Cellular Localisation | Cell MembraneSarcolemmaCytoplasmic Vesicle MembraneTethered To Plasma Membrane And Cytoplasmic Vesicles Via Its Interaction With Phosphatidylserine |
| Alternative Antibody Names | Anti-Tripartite Motif-Containing Protein 72 antibodyAnti-Mitsugumin-53 antibodyAnti-Mg53 antibodyAnti-Trim72 antibodyAnti-Mg53 antibody |
Information sourced from Uniprot.org