• Immunofluorescence analysis of A549 cells using TRIM69 antibody (STJ117821). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using TRIM69 antibody (STJ117821) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TRIM69 antibody (STJ117821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRIM69 antibody (1-341) (STJ117821)

SKU:
STJ117821

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM69 (1-341) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM69
Gene ID: 140691
Uniprot ID: TRI69_HUMAN
Immunogen Region: 1-341
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human TRIM69 (NP_542783.2).
Immunogen Sequence: MEEELAIQQGQLETTLKELQ TLRNMQKEAIAAHKENKLHL QQHVSMEFLKLHQFLHSKEK DILTELREEGKALNEEMELN LSQLQEQCLLAKDMLVSIQA KTEQQNSFDFLKDITTLLHS LEQGMKVLATRELISRKLNL GQYKGPIQYMVWREMQDTLC PGLSPLTLDPKTAHPNLVLS KSQTSVWHGDIKKIMPDDPE RFDSSVAVLGSRGFTSGKWY WEVEVAKKTKWTVGVVRES
Post Translational Modifications Phosphorylated. Phosphorylation is necessary for nuclear localization.
Function E3 ubiquitin ligase that plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis indiana virus. Ubiquitinates viral proteins such as dengue virus NS3 thereby limiting infection. In addition, acts as a key mediator of type I interferon induced microtubule stabilization by directly associating to microtubules independently of its E3 ligase activity. Plays also a role in cataract formation together with TP53. Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination.
Protein Name E3 Ubiquitin-Protein Ligase Trim69
Rfp-Like Domain-Containing Protein Trimless
Ring Finger Protein 36
Ring-Type E3 Ubiquitin Transferase Trim69
Tripartite Motif-Containing Protein 69
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Nucleus Speckle
Adopts A Filamentous Distribution In The Cell Cytoplasm Where It Strongly Colocalizes With Stable Microtubules
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim69 antibody
Anti-Rfp-Like Domain-Containing Protein Trimless antibody
Anti-Ring Finger Protein 36 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim69 antibody
Anti-Tripartite Motif-Containing Protein 69 antibody
Anti-TRIM69 antibody
Anti-RNF36 antibody
Anti-HSD-34 antibody
Anti-HSD34 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance