• Western blot analysis of various lysates using TRIM66 Rabbit polyclonal antibody (STJ11100365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TRIM66 antibody (750-970) (STJ11100365)

SKU:
STJ11100365

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM66 (750-970) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM66
Gene ID: 9866
Uniprot ID: TRI66_HUMAN
Immunogen Region: 750-970
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 750-970 of human TRIM66 (NP_055633.1).
Immunogen Sequence: LATCPLQSIPPVSDMQPETG SSSSSGRTSGSLCPRDGADP SLENALCKVKLEEPINLSVK KPPLAPVVSTSTALQQYQNP KECENFEQGALELDAKENQS IRAFNSEHKIPYVRLERLKI CAASSGEMPVFKLKPQKNDQ DGSFLLIIECGTESSSMSIK VSQDRLSEATQAPGLEGRKV TVTSLAGQRPPEVEGTSPEE HRLIPRTPGAKKGPPAPIEN E
Function May function as transcription repressor.The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association.
Protein Name Tripartite Motif-Containing Protein 66
Cellular Localisation Nucleus
Forms Discrete Foci Within The Centromeric Chromocenter And Surrounding Nucleoplasm
Alternative Antibody Names Anti-Tripartite Motif-Containing Protein 66 antibody
Anti-TRIM66 antibody
Anti-C11orf29 antibody
Anti-KIAA0298 antibody

Information sourced from Uniprot.org

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